Effects of human fibronectin and human serum albumin sequential adsorption on preosteoblastic cell adhesion

Abstract : Fibronectin (Fn) is widely reported to promote cell adhesion and spreading, and recent reports attest to the synergistic effect of coadsorbed albumin (unexpected due to the passivating character of the latter protein). In this study, the sequential adsorption of fibronectin and albumin, and the morphology of cultured MC3T3-E1 preosteoblastic cells are investigated on three important biomaterial surfaces: silicon oxide, poly(styrene) (PS), and hydroxyapatite (HA). Using quartz crystal microgravimetry with dissipation analysis, the adsorbed protein composition and mechanics are determined. Interestingly, cell morphological changes correlate neither with the amount of Fn nor the rigidity of the protein layer. On the PS surface, Alb is seen to significantly diminish cell spreading, possibly due to Alb aggregation with a partially denatured initially placed Fn layer. HA appears to be a particularly favorable substrate for osteoblast adhesion, despite having low Fn adsorption and protein layer rigidity.
Complete list of metadatas

Cited literature [43 references]  Display  Hide  Download

https://hal-u-paris-seine.archives-ouvertes.fr/hal-01926495
Contributor : Rémy Agniel <>
Submitted on : Monday, November 19, 2018 - 11:52:02 AM
Last modification on : Monday, July 1, 2019 - 3:34:08 PM
Long-term archiving on : Wednesday, February 20, 2019 - 2:15:32 PM

File

_pdf_archive_BJIOBN_vol_9_iss_...
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Mathilde Hindi, Emeline Camand, Rémy Agniel, Franck Carreiras, Emmanuel Pauthe, et al.. Effects of human fibronectin and human serum albumin sequential adsorption on preosteoblastic cell adhesion. Biointerphases, American Vacuum Society, 2014, 9 (2), ⟨10.1116/1.4867598⟩. ⟨hal-01926495⟩

Share

Metrics

Record views

39

Files downloads

176